Transcriptomics of L. monocytogenes treated with sodium lactate

L. monocytogenes F2365 strain was grown in Brain Heart Infusion (BHI) liquid broth overnight at 37°C with 200 rpm agitation. The stationary phased bacterial cells were diluted 1:5 in BHI liquid broth supplemented with 4% sodium lactate, grown at 15°C in static for 2 d. Bacterial cells without sodium lactate grown at the same condition (2 d) were used as controls. Bacterial RNA was isolated using RiboPure RNA Purification Kit (Thermo Fisher Scientific). rRNA was depleted from total RNA using Ribo-Zero Magnetic Kit (Illumina). For fragmentation and library preparation, Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) was used according to the manufacturer’s instruction. RNA and DNA quality and library quantification were performed using using a 2100 Bioanalyzer (Agilent Technologies). Sequencing was carried out using Ion Chef and Ion Torrent PGM instruments (Thermo Fisher Scientific).