GPDB

This FAIRsharing record describes: GlycoProtDB is a glycoprotein database providing information of Asn (N)-glycosylated proteins and their glycosylated site(s), which were constructed by employing a bottom-up strategy using actual glycopeptide sequences identified by LC/MS-based glycoproteomic technologies. The database is searchable using gene ID, gene name, and its description (protein name) as query. Each data sheet of glycproteins is based on a single amino acid sequence in Wormpep database for C.elegans and NCBI Refseq database for mouse. The sheet presents actually detected N-glycosylation site(s) which are displayed each capturing methods of glycopeptide subset, e.g., lectins Concanavalin A, wheat germ agglutinin (WGA), or HILIC (hydrophilic interaction chromatography), as well as potential N-glycosylation sites (NX[STC], X?P). Protein sequences, which have common glycopeptide sequence(s), are linked each other.

本FAIRsharing条目描述如下：GlycoProtDB是一款糖蛋白数据库，收录天冬酰胺（Asn，N）连接糖基化蛋白及其糖基化位点的相关信息。该数据库采用自下而上策略构建，依托基于液相色谱-质谱（LC/MS）的糖蛋白质组学技术所鉴定得到的真实糖肽序列进行搭建。该数据库支持以基因ID、基因名称及蛋白描述信息（即蛋白名称）作为查询词进行检索。各糖蛋白数据表均基于单一氨基酸序列，其中秀丽隐杆线虫（C. elegans）的数据取自Wormpep数据库，小鼠的数据则取自NCBI参考序列（RefSeq）数据库。数据表会展示实际检测到的N-糖基化位点，这些位点会根据糖肽亚组分的各类捕获方法进行分类呈现，例如凝集素伴刀豆球蛋白A（Concanavalin A）、小麦胚芽凝集素（wheat germ agglutinin, WGA），或亲水作用色谱（hydrophilic interaction chromatography, HILIC）；同时还会标注潜在的N-糖基化位点（NX[STC]，其中X不为脯氨酸）。拥有共有糖肽序列的蛋白序列之间将建立关联链接。