HCVpp entry in receptor-complemented target cells.

aHCVpp entry of genotype 1a (H77) harbouring the GFP marker gene.
(++), titres higher than 105 IU/ml;
(+), titres between 103 and 105
IU/ml; (±), titres between 102 and
103 IU/ml; (−), titres lower than
102 IU/ml, which corresponds to the threshold of
detection of infected cells by FACS analysis.
bDetection of human CD81 using JS-81 antibody on the surface of the
indicated cells by flow cytometry. (−), MFI (mean
fluorescent intensity) shift of 1; (+), MFI shift between 1
and 50; (++), MFI shift over 50.
cDetection of human Claudin-1 (CLDN1) in lysates of the indicated
cells by immuno-blotting with mouse anti-Claudin-1 antibodies
(Interchim). (−), signal intensity 5-fold lower than that
detected in Huh-7 cells; (+), signal intensity between
5-fold lower and 5-fold higher than that detected in Huh-7 cells;
(++), signal intensity 5-fold higher than that
detected in Huh-7 cells.
dDetection of human SR-B1 using the CLA-1 antibody on the surface of
the indicated cells by flow cytometry. (−), MFI shift of
less than 2; (±), MFI shift between 2 and 6;
(+), MFI shift between 6 and 20; (++),
MFI shift over 20.