Thermal Unfolding of Apo-CaM in increasing ionic strength.
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aData of apoCaM were fit to a three-state model of unfolding where ΔCp was fixed at zero. Data of holoCaM (+Ca2+; from Figure 2) was fit to a two-state model of unfolding with the assumption that C-terminal of holoCaM is already unfolded at 95°C.
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2015-12-02



