Analysis of the m6A methylome and downstream targets of piRNA AVCAPIR in human valvular interstitial cells

To further elucidate the molecular mechanism by which piRNA AVCAPIR drives aortic valve calcification, we performed m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) combined with input RNA sequencing (RNA-seq) in human valvular interstitial cells with or without AVCAPIR overexpression. Overall design: Human valvular interstitial cells from calcific aortic valve disease patients were transfected with piRNA AVCAPIR mimic or negative control (NC) mimic. Following the confirmation of overexpression, cells were sent for m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) combined with input RNA sequencing (RNA-seq).