Transcriptional response of Arabidopsis seedlings to light

We report the transcriptomic response of Arabidopsis thaliana seedlings grown in continuous white light, darkness or darkness and 1 hour of red light Plants were germinated in true-dark conditions as described in (Leivar et al., 2008). Briefly, seeds were sterilized, plated in MS medium without sucrose, irradiated for 3h with white light to induce germination, followed by a saturating far-red light pulse during 5 min before being stratified for 4 days at 4ºC in darkness. They were grown in the dark at 21ºC for 2 days before being collected (D samples) or grown in the dark for 47 hours and treated with 10 μE⋅m−2⋅s−1 red light for 1 hour (R1 samples). White-light grown samples (WLc) were grown under continuous white-light (100 μE⋅m−2⋅s−1 PAR) after stratification for 2 days.