Local temporal Rac1-GTP nadirs and peaks restrict cell protrusions and retractions

Dataset used in the paper "Local temporal Rac1-GTP nadirs and peaks restrict cell protrusions and retractions".The GTPase4.rar file contains the raw confocal images of HT1080 cell line stably expressing RhoA-2G or Rac-2G biosensors seeded on 6.9kPa polyacrylamid gel. Channel 1 and 2 are the FRET signals (excite 457nm, emission 482/35 nm and 525/50 nm). Channel 3 is the red fluorescent beads for the traction force calculation (by comparing to the corresponding cell free image file (containing "RL" in the file name)). Channel 4 is the transmission light for the cell morphology. The Optogenetic.rar file contains the raw confocal images of HT1080 cell line stably expressing PA-Rac1(Q61L) or PA-Rac1(T17N) constructs perturbed by 488 nm light before and after 5 min imaging on the 6.9kPa polyacrylamide gel. Channel 1 is the PA-Rac1 constructs signal. Channel 2 is the far red fluorescent beads for the traction force calculation(by comparing to the corresponding cell free image file (containing "RL" in the file name)). The 3D collagen folder contains the representative raw confocal images of same stable HT1080 cell line seeded in the 3D collagen perturbed by 488 nm light before and after 5 min imaging. The LLSM.rar file contains the representative lattice light sheet images of the HT1080 cell line stably expressing RhoA-2G or Rac1-2G biosensors seeded on the 6.9kPa polyacrylamide gel. The file is in .ims format which could be opened with Imaris software. The WindowingResults.rar file contains the window sampled results from the GTPase4.rar and Optogenetic.rar.The Codes.rar file contains the software and custom codes used in this study.

该数据集收录于论文《局部时序的Rac1-GTP谷底与峰值限制细胞突起与回缩》。GTPase4.rar文件包含了稳定表达RhoA-2G或Rac-2G生物传感器的HT1080细胞系在6.9kPa聚丙烯酰胺凝胶上的原始共聚焦图像。通道1和2为荧光共振能量转移信号（激发波长457nm，发射波长分别为482/35 nm和525/50 nm）。通道3为红色荧光微球，用于牵引力计算（通过与相应的无细胞图像文件（文件名中包含“RL”）进行比较）。通道4为透射光，用于观察细胞形态。Optogenetic.rar文件包含了稳定表达PA-Rac1(Q61L)或PA-Rac1(T17N)结构变异体在6.9kPa聚丙烯酰胺凝胶上经488 nm光照射前后的原始共聚焦图像。通道1为PA-Rac1结构变异体信号。通道2为远红荧光微球，用于牵引力计算（通过与相应的无细胞图像文件（文件名中包含“RL”）进行比较）。3D胶原文件夹包含相同稳定HT1080细胞系在3D胶原上种植并经488 nm光照射前后的代表性原始共聚焦图像。LLSM.rar文件包含了稳定表达RhoA-2G或Rac1-2G生物传感器的HT1080细胞系在6.9kPa聚丙烯酰胺凝胶上的代表性晶格光片图像。文件格式为.ims，可使用Imaris软件打开。WindowingResults.rar文件包含了从GTPase4.rar和Optogenetic.rar中抽取的窗口采样结果。Codes.rar文件包含本研究中使用的软件和自定义代码。