Monomethylation of Lysine 27 at Histone 3 Confers Lifelong Susceptibility to Stress

Histone post-translational modifications are critical for mediating persistent alterations in gene expression. By combining unbiased proteomics profiling, and genome-wide approaches, we uncovered a role for mono-methylation of lysine 27 at histone H3 (H3K27me1) in the enduring effects of stress. Specifically, mice exposed to early life stress (ELS) or to chronic social defeat stress (CSDS) in adulthood displayed increased enrichment of H3K27me1, and transient decrease in H3K27me2, in the nucleus accumbens (NAc), a key brain-reward region. Overall design: Cleavage Under Targets & Release Using Nuclease (CUT&RUN) followed by DNA-sequencing for IgG as well as for histone modifications H3K27me1 and H3K27me2 in nucleus accumbens of adult male mice