Deep mutational scanning of germline and somatic CR9114 by yeast display and Tite-Seq

The study employed deep mutational scanning, which combines saturation mutagenesis and next-generation sequencing, to characterize both germline and somatic variants of the antibody CR9114. This analysis was conducted using yeast display and Tite-Seq. The goal was to evaluate the binding affinity of single amino-acid mutations against influenza A H1 and H3 HAs as well as influenza B HA.