Mass spectrometry identity matches for significant spots.
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Matched fragments; the number of peptides matched with the protein/the total amount of peptides in the sample. Sequence coverage; the percentage of the total protein that the matched peptides cover. Score; the score given by MASCOT, for MS results a score above 66 (56 for actin) was considered significant (p = 0.05), for MS/MS the significance level was a score of 34–37 (protein dependent). Mascot: http://www.matrixscience.com. Uniprot: http://www.uniprot.org.
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2015-12-02



