Human VSMC infected with Ad-CEMIP and immunoprecipitated with anti-CEMIP for proteomic analysis
收藏国家人口健康科学数据中心2026-06-06 收录
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HASMCs were infected with Ad-CEMIP. Whole cell lysates were obtained from 5×108 cells. CEMIP or IgG antibodies are added to capture CEMIP complexes in cell lysates. Agarose beads were washed with IP buffer, CEMIP antibody-binding complexes were captured by adding agarose beads to cell lysates, and non-specific binding was washed with IP buffer. The agarose beads were eluted with 1xSDS for mass Spectrometry.
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北京蛋白质组研究中心创建时间：
2025-03-20
