gene expression profile analysis of in tumor-infiltrating dendritic cells (TIDC) from stress mice compared with Ctrl mice

Social defat did modify the “quality” of TIDC as manifested by massive changes in thegene expression profile. RNAseq analyses revealed signs of SD-induced localimmune suppression, as indicated by the downregulation of myeloid leukocyte differentiation, antigen signaling pathway, neutrophil chemotaxis, cell adhesion, inflammatory response stress and IFN-gamma production. Gene ontology (GO) analysis also suggested that SD amplified the circadian rhythm and cellular metabolism networks in tumor-infiltrating DC. More importantly, SD significantly augmented the level of mRNA encoding glucocorticoid-inducible transcriptional regulator Tsc22 domain family protein 3 (Tsc22d3, also known as glucocorticoid-induced leucine zipper, Gilz) , which functions as a potent mediator of anti-inflammation and immunosuppression. In addition, SD modulated the transcription of a large set of Tsc22d3 downstream target genes identified by Ingenuity® Pathway Analysis.