Antigen exposure reshapes chromatin architecture in central memory CD8+ T cells and imprints enhanced recall capacity [CUT&RUN]

Compared with naïve CD8+ T cells, antigen-experienced effector and central memory CD8 T cells show extensive CTCF redistribution and 3D genome reorgainzation, which underlie the transcriptomic diversification as well as recall capacity in response to secondary challenges. Overall design: P14 CD8+ T cells were first transferred into B6.SJL mice followed by LCMV-Arm infection. On day 8 post-infection, KLRG1+IL-7Ra– P14 cells were sorted as effector CD8+ T (Teff) cells, and After =30 days, CD62L+ P14 cells were sorted as central memory (Tcm) cells were sorted. The sorted cells were subjected to CTCF CUT&RUN analysis.