RNASeq of bone lining niche cell components from healthy vs. myeloma-bearing mice

We compare gene expression in FACS-sorted bone-lining cell components: stromal progenitors, osteoprogenitors and endothelial cells, from myeloma-bearing vs. from healthy mice, and also between myeloma cells flushed from the central bone marrow vs. myeloma cells digested from the bone surface (myeloma-bearing mice only). The myeloma model used is the 5TGM1 murine myeloma cell line, injected into immunocompetent KaLwRij mice. Overall design: Comparative gene expression differences in 100-cell aliquots of sorted bone marrow niche cell types. N = 4 or 5 biological replicates per cell type (from different mice) per condition (healthy or myeloma-bearing).