A maximum of two readily releasable vesicles per docking site at a cerebellar single active zone synapse

Recent research suggests that in central mammalian synapses, active zones contain several docking sites acting in parallel. Before release, one or several synaptic vesicles (SVs) are thought to bind to each docking site, forming the readily releasable pool (RRP). Determining the RRP size per docking site has important implications for short-term synaptic plasticity. Here, we take advantage of recently developed methods to count the number of released SVs at single glutamatergic synapses in response to trains of action potentials. In each recording, the number of docking sites was determined by fitting with a binomial model the number of released SVs in response to individual action potentials. After normalization with respect to the number of docking sites, the summed number of released SVs following a train of action potentials was used to estimate of the RRP size per docking site. To improve this estimate, various steps were taken to maximize the release probability of docked SVs, the..., Collected using HEKA amplifiers, patchmaster software.
Analysed using Igor., , # A maximum of two readily releasable vesicles per docking site at a cerebellar single active zone synapse

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Here we make publicly available the data and analysis results used in this paper. Electrophysiological recordings of simple synapses in mouse cerebellar slices were deconvolved using Igor to obtain SV counts.

Data: FigforN --- Igor file with variance mean and binomial individual and group data based on SV counts