RNAseq of Anopheles gambiae mosquito lines selected for several generations with different agrochemical mixtures

Gene expression changes and polymorphisms were investigated in genetically related mosquito lines maintained for 30 generations under four different selection regimes. All mosquito line derived from the same parental line (Anopheles gambiae Tiassale line from Ivory coast). Selection regimes were as follows: i) no selection pressure (Control line), ii) selection at each generation at larval stage with a mixture of insecticides used against agricultural pests (Ins line); iii) selection at each generation at larval stage with a mixture of herbicides and fungicides used on crops (Herb line), iv) selection at each generation at larval stage with a mixture of both insecticides and non-insecticide compounds (compounds ii + iii, Mix line). The transcriptome of each line was sequenced using RNA-seq with four replicates per condition.