Table_1_HPV prevalence and genotype distribution in 2,306 patients with cervical squamous cell carcinoma in central and eastern China.DOCX

BackgroundTo explore the positivity rate and genotype distribution of human papillomavirus (HPV) in cervical squamous cell carcinoma (CSCC) tissues in central and eastern China and to provide theoretical basis for cervical cancer screening and prophylactic HPV vaccine development in China.MethodsDNA was extracted from paraffin-embedded tissues of CSCC samples and exfoliated cervical cells of cervical cancer screening populations. 23 HPV genotypes were detected by combining polymerase chain reaction (PCR) and reverse dot hybridized gene chip detection technology in 2,306 CSCC tissues and 10,245 cervical cancer screening populations. The genotype distribution of HPV infection was analyzed.ResultsThe overall infection rate of HPVs in 2,306 CSCC patients was 92.71%. The frequency of single-type HPV infection and multiple-type HPV infection were 86.48% and 13.51%, respectively. The most common HPV genotypes detected in Chinese CSCC tissues were HPV-16, HPV-18, HPV-31, HPV-33, HPV-45, HPV-52, HPV-58, and HPV-59. The overall positivity rate of these eight high-risk HPV (HR-HPV) genotypes in HPV-positive CSCC was as high as 96.91%. Of which the positivity rate of seven HR-HPV genotypes related to nine-valent HPV vaccines in HPV-positive CSCC was 95.09%. Meanwhile, the overall infection rates of HR-HPV and low-risk HPV (LR-HPV) in female aged 35–64 years who underwent cervical cancer screening were 13.16% and 1.32%, respectively. The high-frequency HR-HPV genotypes in cervical cancer screening women were HPV-52, HPV-58, HPV-16, HPV-53, HPV-68, HPV-39, HPV-51, and HPV-56, with positivity rates of 2.25%, 1.60%, 1.31%, 1.22%, 0.93%, 0.92%, 0.78%, and 0.74%, respectively.ConclusionAmong women screened for cervical cancer in China, detecting the 8 high-frequency HR-HPV genotypes can reduce technical difficulty and reagent costs, while also improving the efficiency and effectiveness of cervical cancer screening. HPV genotyping assists gynecologists in assessing the risk of HR-HPV-positive cervical intraepithelial neoplasia and guiding them in implementing appropriate interventions. Furthermore, HPV genotyping is helpful for doctors to follow up HR-HPV-positive women and to evaluate the protective effect of HPV vaccine.

本研究旨在探究中国中部和东部地区宫颈鳞状细胞癌（CSCC）组织中人乳头瘤病毒（HPV）的感染率及基因型分布，并为我国宫颈癌筛查及HPV预防性疫苗的研发提供理论依据。研究方法为：从CSCC样本的石蜡包埋组织中提取DNA，并对宫颈癌筛查人群的宫颈脱落细胞进行检测。通过聚合酶链反应（PCR）与反向点杂交基因芯片检测技术相结合，对2,306例CSCC组织样本和10,245例宫颈癌筛查人群的样本进行23种HPV基因型的检测。结果分析显示，2,306例CSCC患者中HPV的整体感染率为92.71%，其中单型HPV感染和多重型HPV感染的比例分别为86.48%和13.51%。在中国CSCC组织中检测到的最常见HPV基因型依次为HPV-16、HPV-18、HPV-31、HPV-33、HPV-45、HPV-52、HPV-58和HPV-59。这八种高风险HPV（HR-HPV）基因型在HPV阳性CSCC组织中的总阳性率高达96.91%，其中与九价HPV疫苗相关的七个HR-HPV基因型的阳性率为95.09%。同时，在35-64岁接受宫颈癌筛查的女性中，HR-HPV和低风险HPV（LR-HPV）的整体感染率分别为13.16%和1.32%。在宫颈癌筛查女性中，高频率的HR-HPV基因型依次为HPV-52、HPV-58、HPV-16、HPV-53、HPV-68、HPV-39、HPV-51和HPV-56，其阳性率分别为2.25%、1.60%、1.31%、1.22%、0.93%、0.92%、0.78%和0.74%。结论：在中国接受宫颈癌筛查的女性中，检测这8种高频率HR-HPV基因型可以降低技术难度和试剂成本，同时提高宫颈癌筛查的效率和有效性。HPV基因分型有助于妇科医生评估HR-HPV阳性宫颈上皮内瘤变的风险，并指导其实施相应的干预措施。此外，HPV基因分型对医生追踪HR-HPV阳性女性及评估HPV疫苗的保护效果亦具有重要作用。