GNPS - OAE (ocean alkalinity enhancement) project

OAE - These samples are both coral tissue metabolites and water column metabolites collected through needle biopsies and PPL resins from a mesocosm experiment at the Hawaii Institute of Marine Biology. The aim of this experiment is to identify the impact of the carbon sequestration technique ocean alkalinity enhancement on the microbiome and metabolome of the coral holobiont and the surrounding water column.Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 1.7 um 100 A pore size C18 reversed phase UHPLC column 150 x 2.1 mm (Phenomenex, Torrance, CA).