Enterovirus 71 infection induces pyroptotic brain injury via synergistic activation of classical inflammasome and viral gInfection Induces Pyroptotic Brain Injury via Synergistic Activation of Classical Inflammasome and Viral Gasdermin D cCleavage

This dataset, which supports a study investigating enterovirus 71 (EV71) -induced arson brain injury, contains multimodal experimental data for five main illustrations and supplementary material.Data generation and processing: An animal model was established by infecting BALB/c mice (6-8 weeks old) and using uninfected mice as controls. Tissues and samples were collected at multiple time points and performed in parallel in vitro experiments using a human neural cell line (RD). For data generation:Figure 1 includes: (A) raw images of the preparation process of the animal model before sampling; (B) tabulated data of mouse body weight change and disease score assessment (with columns of "time point", "group", "body weight (g)" and "disease score"); (C) original Western blot band images and gray intensity statistics; (D) Raw images with HE and Nissl staining.Figure 2 contains the raw four-color immunofluorescence images and colocalization statistics, which analyze the spatial relationships of target proteins in terms of colocalization coefficients.Figure 3 contains: (A) raw Western blot band images and gray-scale intensity statistics; (B) original immunofluorescence images and optical density statistics of pre-Il-1β + cleaved IL-1β; (C) Raw immunofluorescence images and optical density statistics of VP-1 + IL-18.Figure 4 includes: (A) statistics for body weight and disease score; (B) Raw images with HE and Nissl staining.Figure 5 contains: (A) the original Western blot band image and gray intensity statistics; (B) original immunofluorescence images and optical density statistics of pre-caspase-1 + cleaved caspase-1; (C) Raw immunofluorescence images and optical density statistics of GSDMD + Caspase-11.The supplementary figure presents the original image of the cell state in the in vitro assay.Data processing involved: quantification of Western ink gray scale and immunofluorescence optical density using ImageJ; Statistical software (GraphPad Prism) was used to analyze body weight, disease score, and colocalization/optical density data. Equipment included conjugate focal microscopy (for imaging), gel electrophoresis, and an aspiration system (for Western blotting).There were no omisses or errors, and all experiments were done using three to five biological replisome and technical replicas. Data formats included TIFF (imaging), CSV/Excel (tabular data), all accessible through standard scientific software. The data set made the research on the mechanism of viral encephalitis, neck leakage or the pathology of EV71 reproducible and reusable.