Expression profiling of garlic genes

To characterize the expression of 58219 genes annotated from garlic genome, the transcriptomes of seven tissues (including garlic sprouts, shoots, bulbs, flowers, roots, pesudostems, and leaves) were sequenced, and finally identified 45,750 genes expressed in various tissues investigated. In addition, the bulbs-transcriptomes in the eight stages of bulbs-development were sequenced, resulting in 6,234 genes isentified to show dynamic expression changes in the bulbs-developmental process. Overall design: The garlic Ershuizao cultivated in the experimental farm of the Shandong Agricultural University, Taian, China, in Oct. 2018 was used for the transcriptome analysis. When the garlic buds, the shoots were collected. In May 2019, the leaves, bulbs, pesudostems, garlic sprouts, roots and flowers were individually sampled. In addition, to characterize the expression of expanding bulbs dynamically, bulbs samples from eight stages of growth were collected, by sample each five day from April 11 to May 16 in 2019 when the bulbs have not start the expanding growth and have finished the expansion, respectively, and correspondingly, the sample names were designated orderly from S1 to S8. Three replicates were performed for each sample. Total RNAs for all 42 samples were separately extracted and used to construct the cDNA library with fragment lengths of ~250 bp. Thereafter, paired-end sequencing was performed for these 48 samples using the Illumina HiSeq 2500 sequencing platform.