Transcriptional profiling of bladder carcinoma T24 cells following CD40 activation by membrane-bound CD40L (mCD40L) compared to soluble CD40L (sCD40L)

We utilised the Affymetrix microarray analysis to compare the expression profiling of the CD40-positive bladder carcinoma T24 cells expressing mCD40L delivered by a replication-deficient adenovirus (RAdnCD40L) or treated with sCD40L (1µg/ml) for 24 hours Bladder carcinoma T24 cells were infected with 50 MOI RAdMock (RAd expressing GFP) or RAdnCD40L (RAd expressing noncleavable version of human CD40L) or left uninfected for 24 hours or treated with sCD40L (1 µg/ml) for 24 hours, total RNA was isolated and processed for microarray analysis