Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse nucleus accumbens (jrc_mus-nacc-1)

This acquisition is part of the CellMap 2024 Segmentation ChallengeChallenge DOI: https://doi.org/10.25378/janelia.c.7456966Challenge Website: https://cellmapchallenge.janelia.org/Sample: Wild-type, adult male mouse, strain: C57/BL6J from Charles RiverSample Description: The sample is from a wild-type, adult male mouse, strain C57/BL6J, obtained from Charles River. It underwent chemical fixation and reduced osmium processing, followed by treatment with 2% samarium trichloride and 1% uranyl acetate in maleate buffer, and was subsequently embedded in Durcupan resin. The sample was provided by and prepared for imaging by Richard Weinberg (University of North Carolina), with imaging conducted by C. Shan Xu (Yale) and Kenneth Hayworth (HHMI/Janelia). Post-processing was performed by Gleb Shtengel and Harald Hess (HHMI/Janelia).Protocol: Chemical fixation, reduced osmium processing, treated with 2% samarium trichloride and 1% uranyl acetate in maleate buffer, then embedded in Durcupan resin.Contributions: Sample provided by and prepared for imaging by Richard Weinberg (University of North Carolina), with imaging by C. Shan Xu (Yale), Kenneth Hayworth (HHMI/Janelia), and post-processing by Gleb Shtengel, and Harald Hess(HHMI/Janelia).Acquisition ID: jrc_mus-nacc-1Voxel size (nm): 4 x 4 x 4 (x, y, z)Data dimensions (µm): 9.44 x 9.77 x 2.25 (x, y, z)Imaging start date: 3/11/2015Imaging duration (days): 1Dataset URL: s3://janelia-cosem-datasets/jrc_mus-nacc-1/jrc_mus-nacc-1.zarr/recon-1/em/EM DOI: https://doi.org/10.25378/janelia.27075514Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-nacc-1Publication: Xu et al., 2021