Seasonal patterns in B-vitamins and cobalamin co-limitation in the Northwest Atlantic

B-vitamins are important co-enzymes that have long been hypothesized to play key roles in marine ecosystems. However, environmental measurements remain scarce, which limits our understanding of their potential impact. Here, we present mass spectrometry-based measurements of B-vitamins (B1, B2, B3, B5, B6, B12) and related vitamers along a transect in the Northwest Atlantic Ocean, in both particulate phase and dissolved in seawater, seasonally over 5 years. We then couple these data with targeted investigations of the impact of B12 (cobalamin) on phytoplankton growth. We show that these metabolites are present at femto to pico-molar concentrations and demonstrate that season explains most variance in particulate phase B-vitamins but not dissolved, offering further evidence that metabolite inventories in these two phases are often decoupled. We find positive correlations between particulate organic carbon with particulate B1 and B3, and between chlorophyll a and particulate B2 and DMB in ..., , # **Seasonal patterns in B-vitamins and cobalamin co-limitation in the Northwest Atlantic**

Dataset DOI: [10.5061/dryad.sbcc2frkc](https://cac-word-edit.officeapps.live.com/we/10.5061/dryad.sbcc2frkc)

**Seasonal patterns in B-vitamins and cobalamin co-limitation in the Northwest Atlantic**\
**Authors: C.** Bannon, P. L. White, E. Rowland, K. J. More, A. Gleason, M. Roberts, E. Devred, L. Beazley, J. LaRoche, E. Bertrand\
**Corresponding Author:** Dr. Erin Bertrand\
**Date of Data Collection:** 2016–2020\
**Location:** Northwest Atlantic Ocean

## **Description**

This dataset supports the findings of the above study, which investigates the distributions and ecological roles of dissolved and particulate B-vitamins and other related biochemical parameters in coastal marine environments. Data were collected during research cruises and bottle incubation experiments on and off the continental shelf.

### **Files and variables**

### **File 1:** LO-24-0698_Manuscript_Data_PARTICULATE.csv

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