Gene expression profiling of transgenic d16HER2- and WT HER2-positive transgenic mammary tumor cell lines. Mus musculus

d16HER2 is a splice variant of HER2 receptor characterized by a significant tumor aggressiveness. We derived primary tumor cell lines from spontaneous tumors grown in mice transgenic respectively for human d16 (MI6 and MI7 cell lines) and WT HER2 isoforms (WTHER2_1 and WTHER2_2). To analyze the molecular mechanisms underlying the higher tumorigenicity of d16HER2 than that of WTHER2, we conducted gene expression profiling analysis of these cell lines. Overall design: Gene expression profiling was performed using RNA from two transgenic d16HER2-positive (named MI6 and MI7) and two transgenic WT HER2-positive (named WTHER2_1 and WTHER2_2) mammary tumor cell lines.