Early GABAergic interneuron function in the neonatal hippocampus revealed by opto-tagging in vivo

This dataset contains single unit data acquired in mouse pups from P3 to P12 during active sleep and while optogenetic experiments were performed to activate and identify GABAergic neurons. Matlab data arrays (mfiles) and spreadsheets (excel files) are provided together with the codes used for statistical analyses and figure generation. %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% Systems requirements: Matlab version 2023b or higher (2026 version may not work though) we used windows 11 on a Dell precision 7780, 13th Gen Intel(R) Core(TM) i7-13850HX (2.10 GHz) 32,0 Go RAM Detailed description: Xlsx files: CellData.xlsx contains cell measurements and attributions colmumn values should be self explanatory, please contact us for more info. SessionData.xlsx contains information for each session. Both are used as table arrays in the following matlab codes. .mfiles are codes for the figures and statistics used in the manuscript .mat files contain variables as follows : Raster_BigPSTH.mat : data concerning cell response to eSPWs BigPSTH : peri event histogram (PETH) for all cells aligned to eSPW onset. Each row is the averaged PETH for each cell, columns are time bins (values described in XPSTH) BinZs: the time bin value in ms dat: an array with computed values for each cell. The value information is stored in the ‘header’ variable. Header values should be self explainatory, please contact us for more details. StimPSTH.mat : data concerning cell response to optogenetic stimulation BigPSTH: Peristimulus histogram for all cells recorded in the optotagging sessions. Each row is the averaged PSTH for a given cell. Columns are time bins (values described in XPSTH) Age: the age of the mice when the cell was recorder celId: the cell identification number in the session celnum: the cell number for the whole dataset IsInhib: whether the cell is significantly inhibited (0 or 1) by the stimulation (computed using a paried t-test between baseline and post stimulation period). Optg: whether the cell was optotagged (0 or 1) PrecisePETHs.mat: data for Figure 5E, restricted to Sharp and Sharp+Tail cells only (412 cells), the average spike time during the sharp period 10 (±100 ms around the eSPW trough)? Same as above, plus mean and median spike times. Usage: Put all files in a directory (say in ‘C:\Users\me\codes’) then, with matlab command line, run cd (‘C:\Users\me\codes’) then, for instance: MortetEtAl2026_fig1 Or LME_GLMMs Expected time to run each file: 2 to 5 seconds.