Acesulfame K-fed and control microbiota DataSet

[Description of methods used for collection/generation of data] Pooled-fecal microbiota from 5-7 years old chlidren was inoculated into the three colon reactors (R1, R2 and R3) of the BFBL gut simulator. The stabilized microbiota were inoculated (1%) with the child pooled fecal sample. After the microbiota stabilization stage, acesulfame-K was added to the system at increasing concentrations (0.5 g/L, 1.5 g/L, 3g/L and 5 g/L), three times per day for 7 days for each concentration (samples W2-W5). The experiment without addition of acesulfame-K to the BFBL gut simulator was carried out for the same period of time (Control, samples C). During the experimental set up, samples were collected every 24 h from the three colon reactors and centrifuged (10,000 ×g during 10 min at 4 °C), and the pellet was used for bacterial genomic DNA extraction (EZNA® Bacterial DNA Kit, Omega Biotek) and using a FastPrep equipment for mechanical lysis (Bio 101 FastPrep FP120, Savant Instruments). DNA samples were sent to Novogene Europe (Cambridge, UK) for sequencing the V3-V4 region of the 16S rRNA gene amplified by using 341F (5′- CCTAYGGGRBGCASCAG-3′) and 806R (5′-GGA CTACNNGGGTATCTAAT-3′) primers. The PCR products were sequenced on a paired-end Illumina platform (NovaSeq 6000, Illumina) to generate 250 bp paired-end raw reads. Shotgun analysis was performed with W5 samples (R1, R3; Ace-K).