Celastrol-based nanomedicine hydrogels eliminate posterior capsule opacification - Supplementary table

Aim: To formulate an injectable thermosensitive micelle–hydrogel hybrid system loaded with celastrol
(celastrol-loaded micelle hydrogel: CMG) to prevent posterior capsule opacification (PCO). Materials
& methods: Celastrol-loaded micelles were embedded in a thermosensitive hydrogel matrix to enable
controlled on-demand celastrol delivery into the residual capsule. The efficacy and mechanisms of the
system for eliminating PCO were evaluated in rabbits. Results: Celastrol-loaded micelles inhibited the
migration and proliferation of lens epithelial cells induced by TGF-β1. Celastrol prevents epithelial–
mesenchymal transition in lens epithelial cells induced by TGF-β1 through the TGF-β1/Smad2/3/TEAD1
signaling pathway. In vivo efficiency evaluations showed that CMG demonstrated an excellent inhibitory
effect on PCO in rabbits and had no obvious tissue toxicity. Conclusion: Injectable CMG may represent
a promising ophthalmic platform for preventing PCO. This versatile injectable micelle–hydrogel hybrid
represents a clinically relevant platform to achieve localized therapy and controlled release of drugs in
other disease therapies.

目标：本研究旨在构建一种负载Celastrol（Celastrol负载的微球水凝胶：CMG）的注射用热敏感微球-水凝胶混合体系，以预防后囊膜混浊（PCO）。材料与方法：Celastrol负载的微球被嵌入热敏感水凝胶基质中，以实现按需控制Celastrol向残留囊膜的释放。该系统在兔子身上对消除PCO的疗效和机制进行了评估。结果：Celastrol负载的微球抑制了由TGF-β1诱导的晶状体上皮细胞的迁移和增殖。Celastrol通过TGF-β1/Smad2/3/TEAD1信号通路预防了由TGF-β1诱导的晶状体上皮细胞的上皮-间质转化。体内效率评估显示，CMG在兔子身上对PCO具有显著的抑制作用，且无明显的组织毒性。结论：注射用CMG可能代表了一种预防PCO的具有潜力的眼科平台。这种多功能的注射用微球-水凝胶混合体系是一种具有临床相关性的平台，可用于实现局部治疗和药物在其它疾病治疗中的可控释放。