RNA-sequencing of Smoothened Agonist (SAG)- versus solvent-treated (DMSO) TtT/GF cells

We analysed the transcriptional changes in the murine folliculostellate cell line TtT/GF after treatment with the Hedgehog signaling activator SAG or its solvent control DMSO, respectively. After activation of the Hedgehog signalling cascade the TtT/GF cells start to transcribe genes that are implicated in extracellular matrix formation and some neuropeptides are transcribed. Partially these genes are described to alter the hormone status in endocrine cells. Therefore, our data implicate that Hedgehog signalling can control hormone secretion in the pituitary gland in an indirect way via folliculostellate cells. Overall design: mRNA profiles of Smoothened Agonist (SAG)- versus solvent-treated (DMSO) TtT/GF cells were generated by RNA-sequencing