Comparative impact of Target of Rapamycin (TOR) disruption on the transcriptome of Arabidopsis thaliana and Setaria viridis

RNASeq analysis was performed in whole seedlings of A. thaliana and S. viridis grown hydroponically at equinoctial conditions (12 h light/ 12 h dark) exactly as described in Monte Bello et al. (2018). Nutrient solution of the hydroponic tanks was replaced with fresh medium containing 0.05 % DMSO (control) and 2 μM or 10 μM of AZD-8055 (an active-site TOR inhibitor), for Arabidopsis and Setaria, respectively, 15 min before the beginning of the light period. Samples were harvested at 0 h (before application of treatments) and after 2, 4 and 6 h of exposure to the chemicals. These results are part of the manuscript entitled "Metabolic regulaTOR: shedding light on the dynamic role of the “Target of Rapamycin” kinase in C3 and C4 species.Monte-Bello CC, Araujo EF, Martins MCM, Mafra V, da Silva VCH, Celente V, Caldana C (2018) A flexible low cost hydroponic system for assessing plant responses to small molecules in sterile conditions. J. Vis. Exp. 138: e57800, doi:10.3791/57800.