RASL-SEQ of Cardiac Transcriptomes from RBFOX1 WT, KO and TG. Mus musculus breed:C57BL6/J

RNA splicing is a ubiquitious post-transcriptional process for all multi-exon genes in eukaryotes which contributes to the total complexity of the human transcrtiptome and is important for refining cellular identity and function. It is well established that alternative mRNA splicing affects a broad spectrum of cardiac genes during normal development and disease, yet the underlying molecular mechanisms remain unexplored. Here, we examine the global RNA splicing events in pressure-overload induced failing mouse hearts and identify a muscle-specific isoform of RBFox1 as a key trans-activating RNA splicing regulator. RNA-mediated oligonucleotide Annealing, Selection and Ligation with Next-Generation sequencing (RASL-seq) was used to analyze the role of RBFox1 in alternative splicing. Splicing changes were compared between 6 groups: Control WT, Control TG, Control KO, TAC WT, TAC TG, TAC KO