Changes in cardiac miRNA transcriptome following isoproterenol infusion and coadministration of minocycline.

Mice were infusion with either vehicle or isoproterenol for 21 days. Another group of mice received both isoproterenol infusion and daily minocycline administration for the duration of the experiment. After 21 days mice were assessed for cardiac function and hearts collected to isolate miRNA. 12-week-old male mice received miniosmotic pumps receiving either sterile saline (vehicle control) or isoproterenol at a dose of 30 mg/kg a day. A third group of mice received both isoproterenol and coadministration of minocycline via IP injection at a dose of 50 mg/kg a day. After 21 days mice were euthanized and tissue collected for miRNA isolation and sequecing.