Percentage of anucleate cells and growth rate of suppressors cultivated in slow or fast growth condition.

Cells were grown in LB at 37°C (non permissive) and in MM at 30°C (permissive) to exponential growth phase (OD600nm between 0.3 and 0.6). The percentage of anucleate cells was measured after staining of DNA with DAPI and membranes with FM4-64FX and visualization of cells by microscopy. Number of examined cells is shown in parentheses. Generation time during exponential growth phase of the different strains has been estimated from monitoring optical density (OD600nm) of cultures in rich medium at 23°C and 37°C. To determine ratio of colonies formed per OD600nm unit, cells were grown in rich medium at 23°C or 37°C up to exponential growth phase (OD600nm between 0.3 and 0.6) and then spread on LB plate and incubated 3 to 4 days at 23°C. NA: not applicable. The average from a minimum of two independent experiments is shown.Percentage of anucleate cells and growth rate of suppressors cultivated in slow or fast growth condition.