RNAseq on Dengue specific mice immunoglobulin repertoires

This study aims to understand the characteristics and diversification of Dengue specific immunoglobulin repertoires after different immunization strategies in mouse. We propose two different immunization strategies in dengue vaccine development. One is tetravalent strategy, which pre-mixed four serotype dengue DNA vaccine and administrated four times. The other one is sequential strategy, which sequentially administrated four time, each serotype vaccine for each dose as sequences of Den1, Den2, Den3 and Den4. Overall design: C57BL/6J (B6) mice were injected Dengue Envelope DNA vaccine with either tetravalent or sequential strategy. The Dengue specific B cells were fished out from immunized mice splenocytes by Dengue Env protein probes. After cDNA and Ig specific amplification, the RNA-seq libraries were prepared and subjected to MiSeq V3 2 × 301 bp sequencing.