Genome-wide transcriptome analysis of Epiblast Like Cells (EpiLC), early Primordial Germ Cells (PGCLCd3) and late Primordial Germ Cells (PGCLCd5) with WT or EsrrbKO background

We report the application of genome-wide RNA-sequencing analysis for WT or EsrrbKO mES cells after transition towards EpiLC and subsequent differentiation in early (d3) or late (d5) PGCLC. We find that formative early and formative late gene signatures were significantly reduced in EsrrbKO EpiLC compared to WT EpiLC. Furthermore, both early and late PGC gene sets were significantly downregulated in EsrrbKO PGCLC, while somatic markers were robustly increased. This indicates that the developmental program is not properly activated during transition towards PGCLC in EsrrbKO cells. Overall design: mRNA profiles of WT or EsrrbKO mES during transition towards EpiLC and subsiquent differentiation in early (d3) or late (d5) PGCLC.