Data-mining bioinformatics: suggesting <i>Arabidopsis thaliana</i> L-type lectin receptor kinase IX.2 (<i>LecRK-IX.2</i>) modulate metabolites and abiotic stress responses

The central role of the Arabidopsis <i>LecRK-IX.2</i> gene in response to biotic stress has been well established by an array of workers. So far, the role of <i>LecRK-IX.2</i> in abiotic stresses has not been investigated systematically. Here, we have first investigated a comprehensive <i>in silico</i> survey to explore the regulation, expression pattern in responses to a wide range of abiotic stresses. The present study reveals that the <i>LecRK-IX.2</i> promoter has numerous potential <i>cis</i>-regulatory elements (CREs) that are regulated by different stresses. AtGenExpress data elucidate that <i>LecRK-IX.2</i> gene plays a potential role in responses to cold, osmotic, drought, salt, UV-B, heat, wound, and genotoxic compound. The expression profile of the co-expressed genes suggests that Arabidopsis <i>LecRK-IX.2</i> gene might have a potential role in stress responses in a tissue-specific manner. Furthermore, a probable signal transduction mechanism has been described by using protein–protein interaction (PPI) dataset. Moreover, the present data-mining investigations have suggested that <i>LecRK-IX.2</i> gene modulates cellular metabolites and abiotic stress responses.拟南芥（Arabidopsis）LecRK-IX.2基因在应对生物胁迫中的核心作用已被多项研究充分证实。截至目前，该基因在非生物胁迫中的功能尚未得到系统探究。本研究首次通过全面的计算机模拟（in silico）分析，探索了其在多种非生物胁迫响应中的调控机制与表达模式。研究发现，LecRK-IX.2基因启动子区域存在大量受不同胁迫调控的潜在顺式调控元件（cis-regulatory elements，CREs）。AtGenExpress数据库数据显示，LecRK-IX.2基因在应对低温、渗透胁迫、干旱、盐胁迫、UV-B辐射、高温、机械损伤以及遗传毒性化合物胁迫中具有潜在功能。共表达基因的表达谱提示，拟南芥LecRK-IX.2基因可能以组织特异性方式参与胁迫响应过程。此外，本研究借助蛋白质相互作用（protein–protein interaction，PPI）数据集，阐明了其潜在的信号转导机制。同时，本次数据挖掘研究表明，LecRK-IX.2基因可调控细胞代谢过程与非生物胁迫响应。