Hyposalinity mannitol experiments conducted with field samples at AWIPEV station

Treatment details – temperature × salinity (Sal) & temperature × nutrients (Nut)initial à 4°C, control salinity, after 2 days of wound healing, ½ PEStemperature acclimation à increase of 2°C every second day, ½ PESset-up Sal:-> 4 temperatures, 2 salinity treatments, 2-L aerated Kautex bottles, ½ PES, 30 μmol m^-2^ s^-1^ PAR, 24-h-light conditions, medium changed 2x/week-> 4°C/low, 4°C/control, 6°C/low, 6°C/control, 8°C/low, 8°C/control, 10°C/low, 10°C/control for 8 daysset-up Nut:-> 4 temperatures, 2 nutrient treatments, 2-L aerated Kautex bottles, 30 μmol m^-2^ s^-1^ PAR, 24-h-light conditions, medium changed every day-> 4°C/SW, 4°C/SW+N+P, 6°C/SW, 6°C/SW+N+P, 8°C/SW, 8°C/SW+N+P, 10°C/SW, 10°C/SW+N+P for 8 days