Results of cotransformation of B. cinerea with pTEL-Fen, sdhB272-sgRNA-RNP and 500bp sdhB-272 (x20) repair template.

1 2x107 protoplasts were transformed with 10μg pTEL-Fen, 6μg Cas9-2μg sdhB272-RNP, and 10μg sdhB-272(x20) repair template. 2To determine the fraction of edited transformants, DNA of individual transformants was prepared, amplified with primers TL151_SdhB_OS_F/ TL152_SdhB_OS_R covering the edited region, and digested with either XhoI (cut in WT sdhB DNA) or XbaI (cut in edited sdhB DNA). n.a.: not analysed. (XLSX)