(A) Morphology of the 253G1 cells expanded on laminin-521 in Essential 8 or mTeSR1 medium after dissociation into single cells. Scale bars, 500 µm. (B) Quantification of the number of dissociated 253G
Juveniles were dissociated in pools of 15 polyps. Polyps were continuously labeled with EdU (100uM, 2%DMSO) before dissociation, and fixed using Trypsin/formaldehyde. Cells were stained with 1μg/ml Fx
