A multi-omics dataset of C57 BL/6J mice regulated function by feed with ε-polylysine

The proteomic and metabolomic differences in mice fed ε-polylysine were detected by high-throughput sequencing and screening. The effects of ε-polylysine on the proteome and metabolomics of C57 BL/6J mice were studied. DIA proteome sequencing and liquid chromatography-mass spectrometry (LC-MS) were used to compare ε-polylysine-modified mouse proteome and metabolome. In this study, we analyzed the regulatory effects of ε-polysine on glycerophospholipid metabolism, glutathione metabolism and ABC transporters pathways. The sequencing data and LC-MS/MS raw data of samples are stored in MetaboLights and ProteomeXchange databases for efficient reuse and to explore the differences in metabolism and protein expression among mice fed with different levels of ε-polylysine, aiming to screen for critical proteins and differential metabolites in valuable regulatory pathways. Mice in each group (n=20) were fed the same diet (AIN-76A) supplemented with 0 (group A), 300 (group B), 600 (group C), and 1,200 (group D) mg/kg ε-polylysine, respectively.

Liver metabolomics is reported in the current study MTBLS4273.
Plasma metabolomics is reported in MTBLS4270.