Exploring the connection between autophagy and heat-stress tolerance in Drosophila melanogaster

Mechanisms aimed at recovering from heat-induced damages are closely associated with the ability of ectotherms to survive exposure to stressful temperatures. Autophagy, a ubiquitous stress-responsive catabolic process, has recently gained renewed attention as one of these mechanisms. By increasing the turnover of cellular structures as well as the clearance of long-lived protein and protein aggregates, the induction of autophagy has been linked to increased tolerance to a range of abiotic stressors in diverse ectothermic organisms. However, whether a link between autophagy and heat-tolerance exists in insect models remains unclear despite broad ecophysiological implications thereof. Here, we explored the putative association between autophagy and heat-tolerance using Drosophila melanogaster as a model. We hypothesized that (i) heat-stress would cause an increase of autophagy in flies' tissues, and (ii) rapamycin exposure would trigger a detectable autophagic response in adults and incre..., Confocal Immunofluoresence Imagery of lysotracker signal in the Drosophila gutWestern blotting against autophagy markersSurvival assays of Drosophila flies, , # Title of Dataset

Exploring the Connection between Autophagy and Heat-Stress Tolerance in Drosophila melanogaster

## General description: files include

Files include:
(1) Survival and developemental data.zip:
\- Developmental\_assay\.txt
\- Survival\_assays\_37\.txt
\- Survival\_assays\_41\.txt:
\- Recovery\_assays\_41\.txt
(2) Immunofluoresence data.zip:
\- Control folder
\- 50 μm rapamycin folder
\- 200 μm rapamycin folder
\- Confocal thresholding\.xlsx
(3) Western blotting data.pdf

Date of data collection: 2019-2023
Localisation of collection: Stellenbsoch
Links to publications that cite or use the data:
Willot, Q., du Toit, A., de Wet, S., Huisamen, E. J., Loos, B., & Terblanche, J. S. (2023). Exploring the connection between autophagy and heat-stress tolerance in Drosophila melanogaster. *Proceedings of the Royal Society B*, *290*, 20231305.

## Description of the data and file structure

(1) Survival and developemental data.zip
\- Developmental\_assay\.txt: Rate of pupa...