Col-HA defect images

Data associated with article: Effects of cell-attachment and extracellular matrix on bone formation in vivo in collagen-hydroxyapatite scaffolds Max M. Villa, Liping Wang, David W. Rowe, and Mei Wei Department of Materials Science & Engineering, 97 North Eagleville Road, University of Connecticut, Storrs, CT 06269 Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 This dataset contains histological sections of critical size calvarial defects filled with collagen-hydroxyapatite scaffolds and culture expanded bone marrow osteoprogenitors after three weeks in vivo. Naming scheme: Group_AnimalEarNotch-Slide#_replicate#Channel#+Channel#+Channel#.jpg example:G1-NB-1_s1c2.jpg Explanation:GroupG1 - samples incubated in vitro overnight before implantationG2 - samples incubated in vitro overnight before implantation +BME gel used as a seeding suspensionG3 -samples implanted immediately after seedingG4 -samples implanted immediately after seeding +BME gel used as a seeding suspension AnimalEarNotchG1&G2NB - ear notch bothNL - ear notch leftNR - ear notch rightHR - hole rightHL - hole leftHB - hole bothG3&G4NB - ear notch bothNL - ear notch leftNR - ear notch rightHR - hole rightHL - hole leftHB - hole bothSlide NumberThree tissue sections were placed on a slide. Slide number can be a value 1, 2 or 3.Replicate numberOne the replicate serial sections of the same calvarium, can be a value s1, s2 or s3.Channel numberCorresponds to 1 of the 3 channels acquired:Channel1-c1- DIC, grayscaleChannel2-c2- calcein mineralization label, greenChannel3-c3- Tdtomato, donor cells, red