Comparison between longissimus dorsi muscle tissue of Fat and Lean Type Pig, among developmental phases

The objective of this study was to identify key genes associated with porcine muscle growth and adipose metabolism which different expression in porcine longissimus dorsi muscle tissue between Fat Type Pig(Taihu pig) and Lean Type Pig (Landrace), among developmental phases(Month 0,1,2,3,4,5). The gene expression analyses will increase understanding of impact factors of pork quality by identifying key genes and pathways controlling longissimus dorsi muscle development. Relative real-time RT-PCR was used to confirm differential expression of 5 different expression genes(PPARGC1A, RYR1, IGF2, IGF1R and IGFBP5) which normalized by 3 housekeep genes(ACTB, TBP and TOP2B). Keywords: time course and breed comparison The different expression experiments between Taihu pig and Landrace used partnership design ( Landrace Month 0 vs Taihu Month 0, 1 vs 1,2 vs 2,3 vs 3, 4 vs 4,5 vs 5). The different expression experiments among developmental phases(Month 0,1,2,3,4,5) used indirect loop design(Landrace Month 1 vs 0, 2 vs 1,3 vs 2,4 vs 3,5 vs 4,0 vs 5; Taihu Pig Month 1 vs 0, 2 vs 1,3 vs 2,4 vs 3,5 vs 4,0 vs 5).