DDX3X Induces Primary EGFR-TKI Resistance Based on Intratumor Heterogeneity in Lung Cancer Cells Harboring EGFR-Activating Mutations - Figure 5

A. Immunoblotting analysis of DDX3X expression in adherent and nonadherent parental PC9 cells and A-1 cells. B. ALDEFLUOR assays were carried out by suspending cells in Aldefluor assay buffer containing 1.5 µM bodipy-aminoacetaldehyde, an ALDH substrate. Cells were then incubated for 45 min at 37°C and analyzed according to the manufacturer’s instructions. C. Flow cytometry analysis of adherent and nonadherent parental PC9 cells and A-1 cells. Histgrams indicate CD44 expression. Dotted lines indicate isotype control, thin lines indicate adherent cells, and the thick lines indicate nonadherent cells. D. Flow cytometry analysis of unfractionated, adherent, and nonadherent cells derived from parental PC9 cells and A-1 cells. X-axis indicates fluorescence intensity of E-cadherin, and Y-axis indicates fluorescence intensity of N-cadherin. E. Flow cytometry analysis of nonadherent cells isolated from parental PC9 and A-1 cells. Histogram plots show vimentin expression on gated N-cadherin+ cells.