Transcriptome-wide effects of cytosine and adenine deaminases on gene expression induced by base editors

We used RNA-sequencing data to explore the transcriptome-wide effects of cytosine and adenine deaminases on gene expression induced by base editors. Overall design: HEK293T cells that expressed only GFP, TadA–TadA* or BE3 and sequenced using Illumina. HEK293T cells that expressed only GFP, TadA–TadA* or APOBEC1 in gradient concentration (1.5ug, 2.5ug, 3.5ug) were sequenced using Illumina. HeLa cells expressed only GFP, TadA–TadA* or BE3 were sequenced using Illumina.