RNA- and Antibody-Based Profiling of the Human Proteome with Focus on Chromosome 19

An
important part of the Human Proteome Project is to characterize
the protein complement of the genome with antibody-based profiling.
Within the framework of this effort, a new version 12 of the Human
Protein Atlas (www.proteinatlas.org) has been launched,
including transcriptomics data for 27 tissues and 44 cell lines to
complement the protein expression data from antibody-based profiling.
Besides the extensive addition of transcriptomics data, the Human
Protein Atlas now contains antibody-based protein profiles for 82%
of the 20 329 putative protein-coding genes. The comprehensive
data resulting from RNA-seq analysis and antibody-based profiling
performed within the Human Protein Atlas as well as information from
UniProt were used to generate evidence summary scores for each of
the 20 329 genes, of which 94% now have experimental evidence
at least at transcript level. The evidence scores for all individual
genes are displayed with regards to both RNA- and antibody-based protein
profiles, including chromosome-centric visualizations. An analysis
of the human chromosome 19 shows that ∼43% of the genes are
expressed at the transcript level in all 27 tissues analyzed, suggesting
a “house-keeping” function, while 12% of the genes show
a more tissue-specific pattern with enriched expression in one of
the analyzed tissues only.