Wetland soil microbial responses to upland agricultural intensification and an invasive macroinvertebrate

Bacteria, archaea, and fungi are dominant microorganisms in wetland soils, which play fundamental roles in determining ecosystem functions and services and are shaped by multiple interacting external forces. To improve understanding of how wetland soil microbiomes are affected by upland agricultural intensification and Pomacea maculata invasion, we surveyed microbial communities using high-throughput sequencing of 16S ribosomal RNA (16S rRNA) and ribosomal internal transcribed spacer (ITS) genes. We specifically ask: (1) how do taxonomic composition shift in soil bacterial, archaeal, and fungal communities along upland agricultural intensification and P. maculata invasion? (2) What are the impacts of water chemistry and soil nutrients on the structure of microbial communities? And (3) how do agricultural intensification and P. maculata invasion affect microbial co-occurrence networks?