Functional dissection of lymphoid progenitor compartments resolves developmental trajectories in B-lymphocyte development

This report describes the identification and characterization of novel lymphoid progenitor populations in the mouse bone marrow. These data resolve the complexity of the classically defined pre-pro-B/FrA cell compartment and provide a model that can be used to develop a unifying view of the progression of early B-cell development. Single cell RNA-seq data were generated on the 10X emulsion platform (10X Genomics, Pleasanton, CA) according to the manufacturer's instructions. The single-cell RNAseq from the Chromium platform was processed using CellRanger v1.3 using default settings, and subsequent normalization, QC, filtering, and differential gene expression analysis was performed in R using Seurat v1.4 (http://www.satijalab.org/seurat). Dimension reduction was performed using DDRTree over three components.