Neopetrotaurines A-C, Isoquinoline Alkaloids with an Unprecedented Taurine-Bridged Isoquinoline Quinone Moiety from the Sponge Neopetrosia sp.

Neopetrotaurines A-C, isoquinoline quinone-linked isoquinoline alkaloids that possess a unique taurine bridge connecting the two bicyclic components, were isolated from a Neopetrosia sp. marine sponge. These new compounds have proton-deficient scaffolds, so their structure elucidation was facilitated by utilizing LR-HSQMBC and HMBC NMR experiments optimized to detect 4- and 5-bond long-range 1H-13C heteronuclear correlations. Neopetrotaurines A-C showed potent inhibition of transcription driven by the oncogenic fusion protein PAX3-FOXO1 in alveolar rhabdomyosarcoma. Overall design: RNA-seq with no replicates. Drug treatment versus control analysis.