A north-south hemispheric migratory divide in butterflies

We prepared ddRAD genomic libraries for 251 Vanessa cardui and 5 Vanessa kershawi individuals following the protocol of Peterson et al. (2012). We used EcoRI and BfaI restriction enzymes and size selection of 300 bp using a PippinPrep instrument and 2% agarose cassettes (Sage Science, Beverly, MA, USA). Libraries were sequenced in 150 bp paired-end reads on Illumina HiSeq 2500 at the Harvard University Bauer Core Facility. We also provide short-reads for a Vanessa kershawi individual. A 350bp insert paired-end library was prepared following the Illumina TruSeq DNA PCR-Free protocol. The library was sequenced at the Swedish National Genomics Infrastructure (NGI) facilities at 150 bp from both ends using a NovaSeq 6000 S4 sequencing platform. Peterson, B. K., Weber, J. N., Kay, E. H., Fisher, H. S. & Hoekstra, H. E. Double Digest RADseq: An Inexpensive Method for De Novo SNP Discovery and Genotyping in Model and Non-Model Species. PLoS One 7, e37135 (2012)