RNAseq analysis of differentiated rat PAC1 pulmonary artery smooth muscle cells (SMCs) compared to proliferative cells, and the RBPMS knockdown and MBNL1&2 knockdown in differentiated PAC1 cells.

Investigation of the regulation of RNA binding proteins RBPMS and MBNL1 via the global-scale analysis of alternative splicing changes in rat artery smooth muscle cells; as well as the alternative splicing changes in differentiated and proliferative PAC1 cells. Bulk mRNA-Seq was carried out after panel knockdown of RBPMS and MBNL1&2 in differentiated PAC1 cells. Overall design: RBPMS and MBNL1&2 were parallel knockdown in differentiated PAC1 cells. RBPMS knockdown was treated with RBPMS-specific siRNA; MBNL1&2 knockdown was treated with MBNL1 and MBNL2 specific siRNA. The differentiated and proliferative PAC1 negative control samples were treated with control siRNA.